Figure 10

Andrographolide (ANDRO) modulates expression of fibrogenic genes in HSC. HSCs were isolated from male Wistar rats as described in material and methods section. Cells were incubated at different time points (24 and 48 hours) with two different AP concentrations (20 and 50 μM). Then, mRNA expression of relevant hepatic fibrogenic genes [Alpha-smooth muscle actin (α-SMA), transforming growth factor beta (TGF-β) and collagen type I, alpha 1 chain (COL1A1)] was assessed. The statistical significance was evaluated using ANOVA with a post-hoc Bonferroni multiple-comparison test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001).