Figure 2 | Scientific Reports

Figure 2

From: Andrographolide Ameliorates Inflammation and Fibrogenesis and Attenuates Inflammasome Activation in Experimental Non-Alcoholic Steatohepatitis

Figure 2

Andrographolide(ANDRO)-treated mice are protected from CDAA-induced liver fibrosis. (A) Liver sections Sirius Red staining from mice fed with choline-deficient amino acid-defined (CDAA) or choline-supplemented L-amino acid defined (CSAA) diets during 22 weeks and treated with ANDRO or Vehicle (V) as described in material and methods. (B) Fibrosis area was quantified using digital image analysis of the red-stained area in Sirius red-stained samples (NIH, Bethesda, MD, USA) and mRNA levels of Collagen, type I, alpha 1 (C), Alpha-smooth muscle actin (α-SMA) (D), matrix metalloproteinase-2 (MMP-2) (E), Tissue inhibitor of metalloproteinase-1 (TIMP-1) (F), transforming growth factor beta TGF-β, (G) and Connective tissue growth factor precursor (CTGF) (H) assessed by real time-PCR. Statistical significance was evaluated using ANOVA with a post-hoc Bonferroni multiple-comparison test (*p ≤ 0.05 and ***p ≤ 0.001. n.d = not detected).

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