Figure 4 | Scientific Reports

Figure 4

From: Andrographolide Ameliorates Inflammation and Fibrogenesis and Attenuates Inflammasome Activation in Experimental Non-Alcoholic Steatohepatitis

Figure 4

Andrographolide (ANDRO) alleviates CDAA-diet-induced inflammation. Mice were fed with choline-deficient amino acid-defined (CDAA) or choline-supplemented L-amino acid defined (CSAA) diets during 22 weeks and treated with ANDRO or Vehicle (V) as described in material and methods. (A) Immunohistochemistry of liver sections using anti-F4/80 antibody, red arrows show inflammatory foci. Hepatic mRNA levels of (B) F4/80, (C) Monocyte chemotactic protein 1 (MCP-1), (D) Tumor necrosis factor-alpha (TNF-α), (E) Interferon gamma (IFNγ), (F) inducible nitric oxide synthase (iNOS), (G) interleukin-10 (IL-10) and (H) arginase-1 (Arg-1) were also assessed by real time-PCR. Statistical significance was evaluated using ANOVA with a post-hoc Bonferroni multiple-comparison test (*p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001).

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