Figure 6 | Scientific Reports

Figure 6

From: Andrographolide Ameliorates Inflammation and Fibrogenesis and Attenuates Inflammasome Activation in Experimental Non-Alcoholic Steatohepatitis

Figure 6

Andrographolide (ANDRO) inhibits IL-1β expression through NFκB inhibition. HepG2 cells were incubated for 24 h with palmitic acid to induce lipid droplet accumulation. Then, cells were stimulated with Lipopolysaccharides (LPS) (1000 ng/ml) for 24 h in the presence or absence of ANDRO (50 μM). (A) interleukin-1β (IL-1β) gene expression. (B) IL-1β protein levels in the supernatant fraction were analyzed by ELISA. (C) Fat-laden HepG2 cells were co-transfected with NFκB transcription factor-responsive firefly luciferase and Renilla luciferase constructs and treated with LPS (1000 ng/ml), ANDRO (50 μM) or both. (D) IL-6 protein levels in the supernatant fraction. Statistical significance was evaluated using ANOVA with a post-hoc Bonferroni multiple-comparison test (**p ≤ 0.01 and ***p ≤ 0.001).

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