Figure 7

Andrographolide (ANDRO) inhibits Caspase-1 (CASP1) activation and interleukin 1β (IL1 β) maturation in vitro. Primary hepatocytes isolated from C57bl6 mice were incubated for 24 h with palmitic acid to induce lipid droplet accumulation. Then, cells were stimulated with Lipopolysaccharides (LPS) (1000 ng/ml) for 24 h in the presence or absence of ANDRO (50 μM). (A) Protein levels of CASP1 and cleaved CASP1 (CASP1-p10) were determined by Western blot as described in material and methods. Actin was used as loading control. The CASP1-P10/CASP1 ratio was calculated to assess the generation of active CASP1. (B) CASP-1 activity as evaluated by an enzymatic assay. Statistical significance was evaluated using ANOVA with a post-hoc Bonferroni multiple-comparison test (*p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001).