Figure 9 | Scientific Reports

Figure 9

From: Andrographolide Ameliorates Inflammation and Fibrogenesis and Attenuates Inflammasome Activation in Experimental Non-Alcoholic Steatohepatitis

Figure 9

Andrographolide (ANDRO) inhibits inducible nitric oxide synthase (iNOS) expression in hepatocytes. iNOS gene expression was evaluated in freshly isolated hepatocytes that were incubated for 24 hours with LPS (A) or a Cytokine Mixture (B) containing TNF-α, Interleukin-1 and interferon-γ in the presence or absence of different concentrations of ANDRO. To evaluate cell viability under the experimental condition a MTS cell proliferation assay (C) and a dose-response curve (D) were performed in these settings. The statistical significance was evaluated using ANOVA with a post-hoc Bonferroni multiple-comparison test (***p ≤ 0.001).

Back to article page