Figure 1
From: Protective and therapeutic effect of felodipine against bleomycin-induced pulmonary fibrosis in mice
Effect of felodipine on TGF-β1-induced collagen production. LL29 cells were incubated with TGF-β1 (5 ng/ml) for 48 h (A, B) or 24 h (C, D) in the presence of indicated concentrations (µM) of felodipine (Fel). Collagen levels in culture medium were determined by Sircol assay (a). Viable cell number was determined by MTT method (b). Total RNA was extracted and subjected to real-time RT-PCR using a specific primer set for each gene. Values were normalised to GAPDH and expressed relative to control sample (c). Whole-cell extracts were analysed by immunoblotting with an antibody against α-SMA or actin (d). The α-SMA band intensity was determined using Image J software (e). Values represent mean ± S.E.M. ** or ## P < 0.01 and # P < 0.05. (* vs Control, # vs TGF-β).
