Figure 3
From: Protective and therapeutic effect of felodipine against bleomycin-induced pulmonary fibrosis in mice
Effect of felodipine on pre-developed pulmonary fibrosis. Mice were intratracheally administered with bleomycin (BLM, 1 mg/kg) or vehicle once only on day 0. Mice were intratracheally administered indicated doses (mg/kg) of felodipine (Fel) once daily for 10 days (from day 10–19). Sections of pulmonary tissue were prepared on day 21 (48 h after final felodipine administration) and subjected to histopathological examination including H&E staining (upper images) and Masson’s trichrome staining (lower images). Scale bar = 1.0 mm (a). Percentage of collagen-positive area was determined based on images of Masson’s trichrome staining (b). Pulmonary hydroxyproline level was determined on day 21 (c). Total respiratory system elastance and FVC were measured on day 21 (d). Values represent mean ± S.E.M. ** or ## P < 0.01; # P < 0.05. (* vs vehicle, # vs BLM).
