Figure 4
From: Protective and therapeutic effect of felodipine against bleomycin-induced pulmonary fibrosis in mice
Effect of felodipine on bleomycin-induced increase in myofibroblasts. Mice were intratracheally administered with bleomycin (BLM, 1 mg/kg) or vehicle once only on day 0. Mice were intratracheally administered indicated doses (mg/kg) of felodipine (Fel) once daily for 3 days (from day 10–12). Sections of pulmonary tissue were prepared on day 13 (24 h after final felodipine administration) and subjected to immunohistochemical analysis with an antibody against α-SMA (a,b). Scale bar = 100 μm. Percentage of area stained with antibody was determined using ImageJ software (b). Values represent mean ± S.E.M. ** or ## P < 0.01. (* vs vehicle, # vs BLM).
