Figure 6
From: Protective and therapeutic effect of felodipine against bleomycin-induced pulmonary fibrosis in mice
Effect of other calcium blockers on bleomycin-induced pulmonary fibrosis, alteration of lung mechanics, and respiratory dysfunction. Mice were intratracheally administered with bleomycin (BLM, 2 mg/kg) or vehicle once only on day 0. Mice were intratracheally administered indicated doses (mg/kg) of nifedipine (Nif) or benidipine (Beni) once daily for 14 days (from day 0–13). Sections of pulmonary tissue were prepared on day 14 (24 h after final calcium blockers administration) and subjected to histopathological examination. H&E staining (upper images) and Masson’s trichrome staining (lower images); scale bar = 1.0 mm (a). Percentage of collagen-positive area was determined based on images of Masson’s trichrome staining (b). Pulmonary hydroxyproline level was determined on day 14 (c). Total respiratory system elastance and FVC were measured on day 14 (d). Values represent mean ± S.E.M. ** or ## P < 0.01 and # P < 0.05. (* vs vehicle, # vs BLM).
