Figure 2

Regional association plot of ss715588567 associated with 14GHSE-DAI03 and expression of the putative candidate gene Glyma.04g209700. (a) The top panel shows the negative log10-transformed P values from a genome-wide scan by using a mixed linear model (MLM) for plant severity taken 3 DAI within the adjacent region of peak SNP ss715588567 plotted against base pair positions (Mb) on soybean chromosome 4. The significance threshold line is distinguished (gray dotted line), and each SNP depicts the extent of linkage disequilibrium in the region based on pairwise values with respect to the peak SNP. The values are indicated using the color intensity index shown. The bottom panel shows all putative genes in the region. The putative candidate gene indicated in green. (b) The expression of Glyma.04g209700, the putative candidate gene for ss715588567, in a modern resistant (blue) and susceptible (orange) cultivar. Error bars shown to indicate significance (p < 0.05).