Figure 3 | Scientific Reports

Figure 3

From: Fibronectin promotes directional persistence in fibroblast migration through interactions with both its cell-binding and heparin-binding domains

Figure 3

Syndecan-4 engagement and PKC activation are not required for high directional persistence on FN. (A) Model of potential syndecan-4-mediated regulation of directional persistence in migration. (B) REF speed and directionality index on FN120-coated substrates (10 μg/ml) were not affected by the presence of soluble Hep2 fragments. Values in presence of Hep2 were compared to the control condition using one-way ANOVA analysis. (C) REF speed and directionality index on FN-coated substrates (10 μg/ml) were not affected by the presence of a recombinant syndecan-4 ectodomain (10 μg/ml). Values were compared using a Mann-Whitney test (Nexp = 3; n = 60). (D) REF speed and directionality index on FN-coated substrates (10 μg/ml) were not affected by the presence of the soluble PKC inhibitor bisindolylmaleimide I (BIM-I). Values in presence of BIM-I were compared to control conditions (same amount of DMSO as used for the highest inhibitor concentration) using one-way ANOVA analysis. Nexp: number of independent experiments. Mean ± s.e.m. are presented. ns: not significant.

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