Figure 4
Soluble heparin reduces fibroblast speed on FN but has only a mild effect on directional persistence. (A) REF speed on FN-coated substrates (10 μg/ml) slightly decreased with increasing concentrations of soluble Hep2 domain. Directional persistence on the other hand did not change. Values in presence of Hep2 were compared to the control condition using one-way ANOVA analysis (Nexp = 3; n = 60). (B) REF speed markedly decreased in presence of soluble heparin on FN-coated substrates (10 μg/ml), whereas directionality index showed a significant decrease at a heparin concentration of 100 μg/ml, but not at 300 μg/ml. Values in presence of heparin were compared to the control condition using one-way ANOVA analysis. Nexp: number of independent experiments. Mean ± s.e.m. are presented. ns: not significant, *P < 0.05, ****P < 0.0001. (C) FN-coated TCPS substrates (10 μg/ml) were pre-incubated with 100 μg/ml heparin for 15 minutes at 37 °C, washed 3 times with PBS and seeded with REF. Cell speed and directionality index did not show significant differences compared to untreated FN-coated substrates. Mean ± s.e.m. are presented (n = 40 cells; Nexp = 2). Values were compared using Mann-Whitney tests.
