Figure 1

Identification of exosome miRNA in Giant Panda breast milk. (A) Visualization of the small neonate-maternal weight ratio of the giant panda. This image was taken by Chengdong Wang. (B) The particle size distribution of the milk exosomes detected using AFM. (C) Western blot analysis showing the enrichment of CD63 and TSG101, and the absence of Tubulin in exosomes (exo), when compared to the cellular fraction lysate of the breast milk (cell). (D) Total RNA was extracted from milk exosomes, and Marker represents the 100 nt marker band. The black arrow indicates the direction of electrophoresis. (E) Classification of giant panda miRNA precursors identified in the present study.