Figure 4 | Scientific Reports

Figure 4

From: Combination of RAD001 (everolimus) and docetaxel reduces prostate and breast cancer cell VEGF production and tumour vascularisation independently of sphingosine-kinase-1

Figure 4

Overexpression of SK1 increases basal P70S6K phosphorylation and VEGF expression. Cell extracts for wild type MDA-MB-231 (MDA/WT) and PC3 (PC3/WT), empty vector-transfected MDA-MB-231 (MDA/Neo) and PC3 cells (PC3/Neo) and MDA-MB-231 and PC3 cells stably transfected with human SK1 (MDA/SK1 and PC3/SK1, respectively) were collected. (A,F) P70S6K phosphorylation was measured using ELISA. (B,G) SK1 expression and VEGF expression (D,I) was determined by qRT-PCR. (C,H) SK1 activity was measured using radiolabelling. (E,J) VEGF protein levels were measured using ELISA. Columns, mean of three independent experiments performed in triplicate; bars, SEM. (*P < 0.05; **P < 0.01; §P < 0.001; ns, not significant, P > 0.05).

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