Figure 5 | Scientific Reports

Figure 5

From: Combination of RAD001 (everolimus) and docetaxel reduces prostate and breast cancer cell VEGF production and tumour vascularisation independently of sphingosine-kinase-1

Figure 5

SK1 overexpression does not affect RAD001-induced VEGF downregulation. Wild type, empty vector- and SK1-overexpressing MDA-MB-231 and PC3 cells were starved overnight then incubated with 0.1% DMSO (Control), 100 nM RAD001 (RAD), 5 nM docetaxel (Doc) and the combination of these drugs (RAD + Doc) for 24 h. (A,D) P70S6K phosphorylation was measured using ELISA. (B,E) VEGF expression was determined by qRT-PCR. (C,F) VEGF protein levels were measured using ELISA. Columns, mean of three independent experiments performed in triplicate expressed as relative to control; bars, SEM. (*P < 0.05; **P < 0.01; §P < 0.001; ns, not significant, P > 0.05).

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