Figure 2
From: Structural and Biochemical Characterization of Poly-ADP-ribose Polymerase from Trypanosoma brucei
Characterization of DNA-binding properties and DNA-dependent activation of TbPARP. (A) The binding of TbPARP constructs (2 µM) to DNA template 1 (0.5 µM) were evaluated with agarose gel electrophoresis. (*) Shows the position of the supershifted DNA and (+) displays the smaller DNA shift. (B) Concentration-dependent analysis of DNA binding to TBP-FL. TBP-FL was titrated against 0.5 µM DNA (templates 1 and 6) and analyzed with agarose gel electrophoresis. (C) DNA dependent activation of TBP-FL, TBP-WRA and TBP-RA using different salt concentrations. Activated DNA was used as a template. (D) DNA dissociation from TBP-FL followed by automodification was evaluated as a function of time with agarose gel electrophoresis either without (upper lane) or with (lower lane) inhibitor EB-47 (10 µM).
