Figure 4
From: Structural and Biochemical Characterization of Poly-ADP-ribose Polymerase from Trypanosoma brucei
Structural properties of TBP-N. (A) CD spectra of TBP-N measured from 190 to 260 nm at 20 °C. (B) Thermal denaturation spectra (190–260 nm) of TBP-N from 20 °C to 90 °C. The arrows indicate the spectral shifts upon increasing temperature. (C,D) Effect of DNA binding to the secondary structure of TBP-N. 21 bp dsDNA (C) and 21 bp ssDNA (D) was titrated to TBP-N with different molar ratios ranging from 2:1 to 1:16 protein:DNA ratio. Bold line shows the spectra of the native protein and thin lines the DNA titrations. (E) SAXS scattering curve of TBP-N. (F) Kratky plot of TBP-N showing the disordered state of the domain.
