Figure 7
From: Structural and Biochemical Characterization of Poly-ADP-ribose Polymerase from Trypanosoma brucei
Localization of fusion proteins in transgenic procyclic parasites overexpressing full-length protein (TBP-FL-eYFP) (A); or only the N-terminus (TBP-N-eYFP) (B) or the protein without the N-terminus (TBP-WRA-eYFP) (C) subjected to hydrogen peroxide (H2O2) treatment. PAR localization was also revealed with anti-PAR polyclonal antibody. DAPI was used to identify nuclear and kinetoplastid DNA. The protein overexpression was induced by tetracycline. The merge panels show localization of TBP-FL-eYFP and TBP-N-eYFP to the nucleus irrespective of hydrogen peroxide treatment, while TBP-WRA-eYFP is not localized to the nucleus even after treatment with hydrogen peroxide. Bar: 5 µm.
