Figure 3
From: Image based Machine Learning for identification of macrophage subsets
Comparison of transcription factor mRNA expression in M1 and M2 macrophages. qRT-PCR analysis of (A) STAT1, (B) SOCS1, (C) IRF5, (D) STAT6, (E) SOCS3, (F) IRF4 relative mRNA expression in M1 and M2 macrophages after 6 days of culture. All values are reported relative to the house-keeping gene GAPDH. Data show mean values ± SEM of 3 independent experiments using macrophages generated from 3 different donors. Statistical significance was assessed using student’s t-test (**p < 0.01, ***p < 0.001).
