Figure 2
From: Targeting glioma stem cells in vivo by a G-quadruplex-stabilizing synthetic macrocyclic hexaoxazole
6OTD inhibits the growth of glioma U251 cells in vitro and in vivo. (A) Growth inhibitory effect of 6OTD on the JFCR39 human cancer cell line panel. Zero in the vertical axis means the mean midpoint value of log [GI50] of 6OTD for all the cell lines, and each bar means deviation of the log [GI50] for each cell line from the mean midpoint value. Thus, cell lines that were relatively sensitive and resistant to 6OTD are indicated with the vertical bars above and below 0, respectively. CNS: central nervous system. Because the GI50 value of SF-295 cells (290 nM) was exactly the same as the average value of all cell lines, the bar for this cell line cannot be seen in the graph (the value is zero). (B) Relationship between sensitivities of the cell lines to telomestatin (TMS) and 6OTD. Black solid line means linear approximation curve for the 39 data plots. Orange and blue symbols indicate CNS- and other organ-derived cancer cell lines, respectively. (C) Antitumor effect of 6OTD on U251 xenografts. Photos of vehicle (top) and 6OTD (center)-treated mice at day 35 (n = 6) are shown. Bottom, dissected tumors after drug treatment. (D) Tumor volume (left), body weight change (center), and tumor weight at day 39 (right) in (C). Day 0 means the starting day of drug administration. Error bars mean SD. **P < 0.01, ***P < 0.005.
