Figure 4
From: Targeting glioma stem cells in vivo by a G-quadruplex-stabilizing synthetic macrocyclic hexaoxazole
6OTD induces a GSC-selective DNA damage response. (A) Immunofluorescence staining of GBM146 (GSCs and NSGCs) exposed to DMSO, 6OTD (100 nM), or TMZ (10 μM) for 3 days. Cells were subjected to immunofluorescence staining with anti-53BP1 (green) and γH2AX (red) antibodies. Nuclear DNA was visualized by DAPI (4′,6-diamidino-2-phenylindole, blue). (B) Quantitative analyses of the ratio of 53BP1 (left), γH2AX (middle), and 53BP1/γH2AX double-positive (right) cells in (A). At least 200 cells were analyzed for each condition (**P < 0.01). Each bar represents the mean ± SD.
