Figure 5
From: Targeting glioma stem cells in vivo by a G-quadruplex-stabilizing synthetic macrocyclic hexaoxazole
6OTD preferentially attacks telomeric DNA in GSCs. (A) iFISH analysis for evaluating telomere dysfunction-induced foci (TIF) in GBM146 cells (GSCs) treated with DMSO, 6OTD (100 nM), or TMZ (10 μM) for 3 days. Cells were labeled with anti-53BP1 antibody and PNA probe for G-rich telomeric DNA. White arrows indicate TIF. (B) Quantitative analyses of the percentage of TIF in all 53BP1 foci in DNA damage-positive cells. At least 100 cells were analyzed for each condition. Each bar represents the mean ± SD (**P < 0.01). (C) Z-stack image of 6OTD (upper) and TMZ (lower)-treated GSCs and consecutive xz images on white lines of those cells.
