Figure 1
From: Cycles of vascular plexus formation within the nephrogenic zone of the developing mouse kidney
Development of the first renal blood vessels. (A) At E10.5, CD31 expressing capillaries (arrowheads) are present adjacent to the ureteric bud (UB) and (B-B”’) the metanephric mesenchyme is surrounded by scattered endothelia. (C,D) By E11.25, Blood vessels had formed a vascular ring around the top of the ureteric bud stalk, and cell projections extended into the metanephric mesenchyme (arrowhead; D is a 3-D rendered version of C). (E) Cartoon representing the different tissue regions discussed in this section. (F) Immunostaining for Six2 in the E11.5 metanephric mesenchyme and peri-Wolffian mesenchyme illustrates that these tissue regions are distinct. (G-G”) By E11.5, blood vessels had begun to invade the metanephric mesenchyme, always entering from a peri-Wolffian mesenchymal region. (H-H’) Blood vessels continue to invade the metanephric mesenchyme by E11.75 from the peri-Wolffian mesenchyme (H’ shows the region in the blue box in H; white arrowheads in H’ show connection between peri-Wolffian and metanephric mesenchymal blood vessels). (I-I”) By E12.5, the metanephric mesenchyme is vascularised throughout. UB, ureteric bud; WD, Wolffian duct; MM, metanephric mesenchyme; PWM, peri-Wolffian mesenchyme. Scale bars: (A–E) = 100 µm; (G–M) = 50 µm.
