Figure 5
From: Cycles of vascular plexus formation within the nephrogenic zone of the developing mouse kidney
Endothelial plexuses form around ureteric bud tips and the cap mesenchyme in vitro. (A) Representative images of an E12.5 + 2 days and (B) + 4 days cultured kidney. (C-C’) 4 days cultured kidney showing that plexuses formed in regions where ureteric bud tips are present, but not in regions devoid of ureteric bud tips. Note in C’ that endothelial plexuses do not form around the epithelia of the developing nephrons (green), they only form around ureteric bud epithelia (blue). (D) Endothelial plexuses formed around cap mesenchymal populations in culture. (E) Ter119+ erythroid cells were present in blood vessels in cultured kidneys. (F) Endothelia formed around cap mesenchymal populations in re-aggregated kidneys. Scale bars: 100 µm.
