Figure 2 | Scientific Reports

Figure 2

From: Reduction in Migratory Phenotype in a Metastasized Breast Cancer Cell Line via Downregulation of S100A4 and GRM3

Figure 2

Genome-wide mRNA expression analysis of TMD cells and BMD cells. Of note, PC = principal component, 2D = 2-dimensional culture, and 3D = 3-dimensional spheroid culture. The double asterisks indicate p < 0.01. (A) Projection of three cell lines in the first/second PC array plane (PC1 and PC2). (B) Projection of S100 proteins and others in the PC gene plane (PC1° and PC2°). Note that the metastasis axis (PC2° axis) is ~20° tilted from the second PC axis (PC2 axis). (C) Representative genes whose mRNA expression levels are significantly different in TMD cells and BMD cells along the metastasis axis. (D) qPCR confirmation of mRNA expression levels of S100A4, SSX1, and CDH12 in TMD cells and BMD cells in 2-dimensional and 3-dimensional (spheroid) cultures. (E) Elevated protein level of S100A4 in TMD cells in 2- and 3-dimensional cultures. Full length blots are presented in Supplementary Figure 1.

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