Figure 3
Effects of S100A4 siRNA in invasion capability and spheroid formation in TMD cells. Of note, NC = non-specific control siRNA, 2D = 2-dimensional culture, and 3D = 3-dimensional spheroid culture. The single and double asterisks indicate p < 0.05 and p < 0.01, respectively. (A,B) Reduction of S100A4 mRNA and protein levels by S100A4 siRNA, respectively. Full length blots are presented in Supplementary Figure 2. (C) Reduction in invasion capability by S100A4 siRNA in TMD cells and BMD cells. (D) Representative images of spheroids by TMD cells, S100A4 siRNA treated TMD cells, and BMD cells in the presence of MC3T3 osteoblast-like cells. (E–G) BMD-like transformation of three spheroid parameters (area, roughness, and circularity, respectively) by S100A4 siRNA.
