Figure 1

Diversities of the entire T cell repertoires and CMV NLV-specific T cell repertoires among five healthy donors. (a) NGS of the T-cell repertoire identifies specific CDR3 amino acid sequences and expression of TCRβ variable (TRBV)- and joining (TRBJ)-region genes. Diversity Index and TCR repertoire plots illustrate the wide range of diversity of the total T cell populations of five healthy donors (V001, V002, V003, V004 and V005). (b) Representative flow cytometric profiles of unstimulated and stimulated PBMCs derived from V001 (HLA-A2-positive and CMV-seropositive healthy donor) for HLA-A2-NLV tetramer-positive cells. PBMCs were stimulated once or twice with autologous antigen-presenting cells pulsed with the peptide NLVPMVATV (NLV) in vitro. The stimulation protocol is detailed in the Methods section. (c) T-cell repertoire plots and Diversity Index of CMV NLV-specific CD8+ T cells sorted from unstimulated and stimulated PBMCs from two healthy donors (V001 and V004; HLA-A2-positive and CMV-seropositive healthy donors, respectively). CMV NLV-specific CD8+ T cell repertories are oligoclonal before and after antigen stimulation.