Figure 4 | Scientific Reports

Figure 4

From: Chronic high fat feeding restricts islet mRNA translation initiation independently of ER stress via DNA damage and p53 activation

Figure 4

Evidence of p53 activation in islets from HFD treated mice. (a) Microarray functional grouping analysis of islets from each diet group. (n = 4) (b) Representative immunofluorescent staining of p53, insulin, and DAPI for islets from each treatment group. (n = 3) (c) Enlarged image of p53 staining in REG and HFD treatment groups. (d) Representative immunofluorescent staining of p21, insulin, and DAPI for islets from each treatment group. (n = 3) (e) qRT-PCR analysis of total islet RNA for Cdkn1a expression. (n = 4) (f) qRT-PCR analysis for Cdkn1a expression in MIN-6 β cells treated with 40 hours of palmitate + a cytokine mix to mimic chronic lipotoxicity. *p < 0.05, **p < 0.01.

Back to article page