Figure 5 | Scientific Reports

Figure 5

From: Chronic high fat feeding restricts islet mRNA translation initiation independently of ER stress via DNA damage and p53 activation

Figure 5

Evidence of DNA damage and oxidative stress in islets from HFD treated mice. (a) Representative immunofluorescent staining of γH2AX, insulin, and DAPI for islets from each treatment group. (b) Representative immunofluorescent staining of 4-hydroxynonenal (4-HNE), insulin, and DAPI for islets from each treatment group. (c) Representative immunofluorescent staining of nitrotyrosine, insulin, and DAPI for islets from each treatment group. (d–g) qRT-PCR analysis of total islet RNA for antioxidant genes, including Gpx1, SOD1, Nrf2, and Ppargc1α. (n = 3) *p < 0.05, **p < 0.01.

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