Figure 3 | Scientific Reports

Figure 3

From: Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass Plasticity

Figure 3

Glucose increases H2O2 levels in INS-1 cells. (A) Time-lapse ratio imaging of rat INS-1 cells following treatment with 18 mM D- or L-glucose at t = 4 min. (B) Quantification of HyPer-3 ratio of the cells shown in panel A. (C) Single-cell quantification of INS-1 cells treated as in panel A, showing heterogeneity in the response to D-glucose from three independent experiments. In 33 out of 74 cells, the HyPer-3 ratio rose almost immediately up to a maximum of ~1.65 in 8–10 minutes after glucose stimulation.

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