Figure 2
Effects of ROS agents on P-gp expression in MCF-7S cells. (A) H2O2 increased P-gp mRNA expression, which could be reversed by NAC (10 mM, 30 min of pretreatment). (B) ROSup increased P-gp mRNA expression, which could be reversed by NAC (10 mM, 30 min of pretreatment). (C) H2O2 (0.25 mM, 24 h) or ADR (0.5 μM, 24 h) enhanced P-gp protein expression, as demonstrated via by flow cytometry analysis and quantified according to mean fluorescence intensity. (D) H2O2 (0.25 mM, 24 h) or ADR (0.5 μM, 24 h) enhanced P-gp protein expression, as shown with laser confocal scanning using an FITC-conjugated anti-P-gp antibody. Scale bar: 20 µm. All of the experiments were conducted in triplicate, and data with error bars are presented as the mean ± SD (n = 3). *p < 0.05; **p < 0.01 vs. control.
