Figure 4
Effect of SLC7A11 over-expression on P-gp expression in MCF-7S and MCF-7R cells. (A) SLC7A11 over-expression reduced the ROS level in MCF-7S and MCF-7R cells. (B) SLC7A11 over-expression inhibited P-gp mRNA expression in MCF-7S and MCF-7R cells. Furthermore, MCF-7S cells that over-expressed SLC7A11 were more sensitive to ADR, compared to MCF-7S cells that has been exposed to a control vector. (C) SLC7A11 over-expression increased the cytotoxic sensitivity of ADR on MCF-7S cells. (D) SLC7A11 over-expression reduced ROS levels and P-gp mRNA expression in MCF-7S cells, which could be reversed by H2O2. (E) SLC7A11 over-expression decreased the function of P-gp, which could be reversed by H2O2 (0.25 mM, 24 h), as indicated by intracellular Rho 123 accumulation in MCF-7S cells. Scale bar: 100 μm. All of the experiments were conducted in triplicate, and data with error bars are presented as the mean ± SD (n = 3). *p < 0.05; **p < 0.01 vs. control.
