Figure 1
From: Iscador Qu inhibits doxorubicin-induced senescence of MCF7 cells
Isc Qu treatment of MCF7 cells abrogates low-dose Dox induced G2/M arrest. (A) MCF7 survival plots upon 72 h monotreatment with: Dox (top left), Isc Qu (top right). MRC5 survival plot upon 72 h monotreatment with Isc Qu (bottom). (B) Changes in cell cycle phase distribution of MCF7 cells upon 72 h treatment with Isc Qu (85 µg/mL), Dox (50 nM) or combination Isc Qu (85 µg/mL) + Dox (50 nM) are shown. After treatment, cells were stained with propidium iodide and analyzed by flow cytometry for cell cycle phase distribution changes. M1- apoptotic cells with DNA content corresponding to sub-G1 fraction; M2- cells with DNA content corresponding to G0/G1 phases; M3- cells with DNA content corresponding to S phase; M4- cells with DNA content corresponding to G2/M phases. (C) Light microscope photographs of morphological changes of MCF7 cells induced by Dox. Magnification, 10X.
