Table 1 Sequences of primers and conditions used in real-time qPCR.
Gene | Forward Sequence | Reverse Sequence | Annealing Temperature (°C) |
|---|---|---|---|
Vimentin | 5′-GACCAGCTCACCAACGACA-3′ | 5′-CTCCTCCTGCAACTTCTCCC-3′ | 65.2 |
Snail | 5′-GTCGTGGGTGGAGAGCTTTG-3′ | 5′-TGCTGGAAAGTGAGCTCTGG-3′ | 66.4 |
Twist | 5′-GCCGGAGACCTAGATGTCATTG-3′ | 5′-CCACGCCCTGTTTCTTTGAAT-3′ | 66.9 |
Oct4 | 5′-CTGCAGAAGTGGGTGGAGGAA-3′ | 5′-CTGCAGTGTGGGTTTCGGGCA-3′ | 68.7 |
Sox2 | 5′-CACCCGCATGTACAACATGAT-3′ | 5′-TCTTAGGATTCTCTTGGGCCA-3′ | 67.7 |
Nanog | 5′-TGGATCTGCTTATTCAGGACAG-3′ | 5′-TGCTGGAGGCTGAGGTATTTC-3′ | 65.4 |
IL-1β | 5′-ACGAACATGTCTTCCGTGAT-3′ | 5′-ACCAGGGATTTTTGCTCTCT-3′ | 62.1 |
IL-6 | 5′-ACCTGGACTTCCTCCAGAAC-3′ | 5′-TTGAGGACTGCATCTTCTCC-3′ | 62.0 |
IL-12 | 5′-TCAAACCAGACCCACCCAAG-3′ | 5′-CACAGATGCCCATTCACTCC-3′ | 65.6 |
IL-10 | 5′-CCAGGATGGTGACTCGACTAG-3′ | 5′-TGGCTCTGCTCTCCCAGAAC-3′ | 65.3 |
IL-4 | 5′-AAGCCCTCAGCTAAGCATGT-3′ | 5′-AGGCATCACAGGCTCAAGTC-3′ | 63.2 |
TGF-β | 5′-GAAGTCTAGCTCGCACAGCA-3′ | 5′-CACGTGCTGCTCCACTTTTA-3′ | 64.0 |
MMP-1 | 5′-AAGATGTGGAGACGGTGCAG-3′ | 5′-CAGTCACTCTCAGCCCGAAG-3′ | 65.2 |
MMP-2 | 5′-ACATACAGGATCATTGGCTACACA-3′ | 5′-CGAAGGCATGAGCCAGGAG-3′ | 64.2 |
MMP-13 | 5′-GCCAGAACTTCCCAACCGTA-3′ | 5′-GTGAAGGGCTGCACTGATCT-3′ | 64.5 |
GAPDH | 5′-TCGGAGTGAACGGATTTGGC-3′ | 5′-CCGTTCTCTGCCTTGACTGT-3′ | 64.4 |
