Figure 3
From: Dengue virus activates cGAS through the release of mitochondrial DNA
DENV infection activates cGAS through the release of mtDNA into the cytosol. (A) cGAMP activity measured in A549 lysates incubated with 1 μg/ml DENV-derived RNA or 1 μg/ml poly(dA:dT). (B) Cellular localization of mitochondria (red) in A549 cells infected with DENV2-PDK53 at MOI 1 for 72 hrs. (C) Fold induction of levels of mitochondria-specific DNA sequences mtCOI, mtND4, and mtCytB present in the cytosol of A549 cells infected with DENV2-PDK53 at MOI 1 and MOI 5 or treated with 4 μg/ml tunicamycin. (D) Percentage of Annexin V positive cells in A549 cells infected with DENV2-PDK53 at MOI 0.1 or MOI 1 for 48 hrs, assessed by flow cytometry. In this figure, *depicts P < 0.05; **depicts P < 0.01 and ***depicts P < 0.001.
