Figure 5 | Scientific Reports

Figure 5

From: HIF-1-mediated suppression of mitochondria electron transport chain function confers resistance to lidocaine-induced cell death

Figure 5

ROS production in response to lidocaine treatment is reduced in RCC4-EV cells in a PDK-1-dependent manner. (a) Graphic depiction of reactive oxygen species (ROS) production in RCC4-VHL cells and RCC4-EV cells exposed to 4 mM lidocaine (n = 3). Data depict the ratio of ROS production in treated cells to compared to that in the mean value of RCC4-VHL cells without lidocaine treatment. RCC4-EV cells were transfected with small interfering RNA (siRNA) targeting pyruvate dehydrogenase kinases 1 (PDK-1) or a negative control (scr). (c) ROS production during treatment with 4 mM lidocaine was assayed. (d) Graphic depictions of caspase-3/7 activity (n = 5). The cellular oxygen consumption rate (OCR) (e) and extracellular acidification rate (ECAR) (f) were measured. Data presented in (a–f) are expressed as mean ± standard deviation (SD). Statistical differences between results were evaluated using the t-test (a,d and e) or two-way ANOVA followed by Dunnett’s test for multiple comparisons (b–d). *p < 0.05 compared to the control cell population. # p < 0.05 compared to the indicated experimental groups.

Back to article page