Figure 6
HIF-1 activation is sufficient to resist lidocaine toxicity in neuronal SH-SY5Y cells. (a) SH-SY5Y cells were exposed to 100 µM nPG, 100 µM DFX, or 1% O2 for 4 h. Whole-cell lysates were immunoblotted (IB) using anti-HIF-1α, HIF-1β, or β-actin antibodies. Experiments were repeated twice and representative blots are shown. (b) Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of SH-SY5Y cells exposed to 100 µM nPG, 100 µM DFX, or 1% O2 for 6 h were measured. (c) SH-SY5Y cells were exposed to 4 mM lidocaine and 100 µM nPG, 100 µM DFX, or 1% O2 for 24 h. Graphic depictions of caspase-3/7 activity (n = 5). (d) SH-SY5Y cells were exposed to 4 mM lidocaine and 100 µM nPG, 100 µM DFX, or 1% O2 for 24 h. Cells were harvested and cell death percentages were analyzed by flow cytometry (n = 3). *p < 0.05 compared to the control cell population. # p < 0.05 compared to the indicated experimental groups.
