Figure 5
From: A Microfluidic Chip for Detecting Cholangiocarcinoma Cells in Human Bile
On-chip cholangiocarcinoma (CCA) cell capture and consequent immunofluorescence (IF) staining with CCA-specific biomarkers (CK7 and CK17). Cells were additionally stained with EpCAM and DAPI/Hoechst. Please note that only cells that presented red (CK7) green,(CK17), and blue (DNA) fluorescence were confirmed to be CCA cancer cells that were captured by EpiEnrich beads. (a) Positive CCA cells; (b) negative samples.
