Figure 3
A yvcK deletion enhances the effect of deletions of ugtP and gtaB genes. Strains were grown on LB medium until OD600 = 0.4. (A) Micrographs of WT strain (168) and mutant strains ΔyvcK strain (SG471), ΔugtP strain (SG571), ΔugtP ΔyvcK strain (SG561). Membranes were stained with FM1-43. (B) Histogram of cell lengths for WT strain (n = 1032), ΔyvcK strain (n = 611), ΔugtP strain (n = 970) and ΔugtP ΔyvcK strain (n = 626). (C) Simplified scheme of the first steps of the glycolipid metabolism. Enzymes involved in this pathways are PgcA: Phosphoglucomutase, GtaB: UTP-glucose-1-phosphate uridylyltransferase, UgtP: Processive diacylglycerol beta-glucosyltransferase. (D) Micrographs of WT strain (168) and mutant strains ΔyvcK strain (SG471), ΔgtaB strain (DKE35670), ΔgtaB ΔyvcK strain (SG562). Membranes were stained with FM1-43. E- Histogram of cell lengths for WT strain (n = 390), ΔyvcK strain (n = 183), ΔgtaB strain (n = 355) and ΔgtaB ΔyvcK strain (n = 270).
