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Figure 3

From: The Anti-Hiv Candidate Abx464 Dampens Intestinal Inflammation by Triggering Il-22 Production in Activated Macrophages

Figure 3

ABX464 decreases macrophage presence and pro-inflammatory cytokine production during acute colitis. (a) Acute colitis was induced as described in Fig. 1a. (b) Colons were analyzed for macrophage content by F4/80 labeling. Representative images of macrophage presence in colons of MC only treated mice (left hand panel), MC and ABX464 treated mice exposed to DSS are presented. (c) Total number of F4/80 + macrophages per colon per mouse are are shown (n = 8 each cohort). (d–f) Colons were taken at the end of the protocol, dissected in the proximal, middle and distal region and maintained in culture for 24 hours. Culture supernatant were analyzed for production of IL-6 (d), TNFalpha (e), and MCP-1 (f) by CBA as described in Methods section.

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