Figure 4
ABX464 treatment restores the expression of cytokine and chemokine signature in the colon of DSS-treated mice. (a) Identification of cytokines and chemokines regulated by ABX464 in colons of DSS-treated mice. RNA samples of from colons were taken at day 11 from mice exposed to DSS-exposure according to the protocol shown in Fig. 1a. Heatmaps show differential gene expression between colons of methylcellulose- and ABX464-treated mice as monitored by RT2 profiler PCR array (Qiagen). Scatterblots correlate gene expression in colons of ABX464-treated mice (a), methylcellulose (MC)- and DSS-exposed mice (b) and ABX464- and DSS-exposed mice (c) versus MC only treated mice. Samples represent the pool of RNA samples of n = 6 for each mouse cohort. (d) The clustergram shows differential gene expression between colons of MC- and ABX464-treated mice as monitored by RT2 profiler PCR array (Qiagen). Controls (Co) are tissue samples from mice not treated with DSS. Arrow heads indicate expression level of IL-22.
