Figure 6
The colitis dampening effect of ABX464 is partially mediated by IL-22 (a) Elevated protein levels of IL-22 are detectable on paraffin sections of colons taken from ABX464 treated mice. Colons were isolated at day 11 from mice exposed to DSS-exposure according to the protocol shown in Fig. 1a. (b) Confocal images of paraffin-sections from a colon of an ABX464-treated mouse as described in Fig. 6a. Section was stained for galactin-3 to identify macrophages (red) and IL-22 (green). Nuclei were stained with Hoechst (blue). The selected images shown are representative. Scale bars represent 100 µm the panel show 4x blow-ups. The cells shown in the blow up are located in the lamina propria between perturbed crypts. (c) C57BL/6 mice (n = 7 each cohort) were subjected to the DSS colitis protocol shown. received orally once a day ABX464 in methylcellulose or methylcellulose (MC) only through gavage. ABX464 and MC-treated were divided into two groups receiving daily 100 µg of either antagonistic anti-IL-22 antibody or isotype control. (d) Weight development in ABX464 and methycellulose (MC) only treated mice during DSS-induced colitis in the presence of either control or anti-IL-22 antibody.
