Figure 1 | Scientific Reports

Figure 1

From: Commensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction

Figure 1

Anc and M6 bacteria-containing phagosomes are negative for the autophagosomal marker LC3 and positive for the late endosomal/lysosomal marker LAMP1. Representative confocal images of infected MΦs. Anc bacteria are shown in yellow and the M6 evolved clone in blue. Scale bar: 5 µm. (A) MΦs were infected with a MOI of 20 of Anc and 120 of M6 bacteria. Cells were fixed at 1 h post-infection and stained with α-LC3 antibody (red) and DAPI (cyan). (B) MΦs infected with a MOI of 20 of Anc bacteria at 1 h post-infection, fixed and stained with α-LAMP1 antibody (red) and DAPI (cyan). (C) MΦs infected with a MOI of 120 of M6 bacteria at 1 h post-infection, fixed and stained with α-LAMP1 antibody (red) and DAPI (cyan).

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