Table 1 Melting temperatures of Joint DNAs.

From: Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi

 

Strains

Origin

SIX3 mutation types

Annealing temperature (arithmetic mean °C  ±  standard deviation)

Joint DNA1

Joint DNA2

Fusarium oxysporum f. sp. lycopersici race 1

MAFF 103036

Japan

None

59.3 ± 0.2

71.6 ± 0.5

 

MAFF 305121

Japan

None

61.1 ± 1.2

72.4 ± 0.4

race 2

JCM 12575

Japan

None

60.7 ± 1.0

71.7 ± 0.3

 

4287

Spain

None

60.4 ± 2.2

73.1 ± 0.3

race 3

Chz1-A

Japan

G121A

53.2* ± 0.4

72.3 ± 0.9

 

KoChi-1

Japan

G121A

53.7* ± 1.1

70.2 ± 0.0

 

FolyA007

Japan

T122A

54.6* ± 1.0

71.9 ± 0.1

 

F240

USA

G134A

60.1 ± 1.0

65.5* ± 1.8

 

40-1

Japan

C146T

61.6 ± 2.0

39.3* ± 0.5

 

KC0071

Japan

C146T

60.2 ± 0.0

40.3* ± 0.5

F. oxysporum f. sp. batatas

MAFF 103070

Japan

—

—

—

F. oxysporum f. sp. conglutinans

Cong:1-1

Japan

—

—

—

F. oxysporum f. sp. nicotianae

ATCC 15645

Greece

—

—

—

F. oxysporum f. sp. radicis-lycopersici

MAFF 103044

Japan

—

—

—

F. oxysporum

Fo304

Japan

—

—

—

F. sacchari

FGSC 7611

USA

—

—

—

Alternaria solani

AS2

Japan

—

—

—

Verticillium dahliae

910312a-3

Japan

—

—

—

plasmid DNA

pSIX3 G137C

 

G137C

59.7 ± 0.1

63.8* ± 0.5

  1. +, Detection −, no detection *, relatively low melting temperature of each joint DNA.