Table 3 Comparison of molecular detection for Fol race 3 from field soil of Kochi prefecture.

From: Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi

Sample

Sampled date

Symptom

Location

LAMP with the universal QProbe

LAMP

PCR

Melting temperature (arithmetic mean °C  ±  standard deviation)

Joint DNA 1

Joint DNA 2

SIX4

SIX3

SIX4

DNA of KoChi-1

—

—

—

53.5 ± 0.2

70.1 ± 0.3

+

+

+

Soil DNA 1

2012.5

Yes

N33°31′92″ E133°21′97.1″

53.4 ± 0.4

70.0 ± 0.1

+

—

+

Soil DNA 2

2013.5

Yes

N33°31′50.28″ E133°22.01′66″

53.8 ± 0.2

69.7 ± 0.4

+

—

—

Soil DNA 3

2012.11

No

N33°31′92″ E133°21′97.1″

—

—

—

—

—

Soil DNA 4

2014.11

No

N33°31′50.28″ E133°22.01′66″

—

—

+a

—

—

  1. +, detection −, no detection a, LAMP products were obtained only once in three replicates.