Figure 3

AddA and btuB riboswitches exhibit contextual sensitivity. (A) The adenine riboswitch had sfGFP introduced directly after the riboswitch replacing its reported ORF (previously eGFP), and also fused to the first 150 bp of the previous ORF. Both constructs were analysed for population and single cell response to 2-aminopurine. Error bars indicate standard deviation of measurements for biological triplicates. Single cell colours; Red (0 µM) Blue (8 µM) Orange (32 µM) Green (250 µM). (B) The btuB riboswitch had eGFP introduced directly after the riboswitch replacing its reported ORF (previously btuB), and also fused to the first 150 bp of the previous ORF. Both constructs were analysed for population and single cell response to adenosylcobalamin. Single cell colours; Red (0 nM) Dark Green (6.25 nM) Light Green (12.5 nM) Orange (25 nM) Blue (50 nm).