Figure 4
From: Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
Ribo-attenuators allow addA riboswitch rational re-engineering: (A) Response of each ribo-attenuator to 0, 8, 32 and 250 µM 2-aminopurine as compared to the fusion construct (orange). Error bars indicate standard deviation of measurements for biological triplicates. Full single-cell data is presented in Supplementary Fig. 6. (B) Fluorescence microscopy of the addA fusion showing clear inclusion bodies and the Att1 ribo-attenuator showing soluble dispersed GFP. White bars represent 1 µm.
