Figure 4 | Scientific Reports

Figure 4

From: Resistin promotes the abnormal Type I collagen phenotype of subchondral bone in obese patients with end stage hip osteoarthritis

Figure 4

The functional impact of resistin on the metabolic activity and bone nodule formation of human primary OA osteoblasts. (A) Alkaline phosphatase activity of human primary OA osteoblasts from the femoral subchondral bone following 2wk stimulation with either resistin (500 ng/ml) or media control. Bars represent mean values ± SEM (n = 4). (B) The fold change in the oxygen consumption rate (OCR) following 24 h stimulation of primary human OA osteoblasts with resistin (500 ng/ml), compared to non-stimulated osteoblasts. OCR values (pmol/min) were normalized to total protein following cell lysis prior to fold change calculation. Bars represent mean values ± SEM (n = 10). (C) Representative image of alizarin red stained mineralised bone nodules following 4 wk stimulation of primary hip OA osteoblasts with either resistin (500 ng/ml) or media control. Alizarin red staining was quantified using Image J software. Bars represent mean values ± SEM (n = 4). (D) Time-course of human primary hip OA osteoblast cell proliferation following stimulation with and without resistin (n = 4).

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