Figure 1

Maximum Likelihood (ML) phylogenetic tree based on the capsid encoding region from nucleotide 701–3013 of the 18 Australian HPeV3 sequences together with the closest related Yamagata 2011 virus sequences. Sequences were aligned using Clustal W and phylogenetic analysis was conducted using MEGA 6 and the Maximum Likelihood (ML) method based on the Tamura-Nei model⁵¹. The tree with the highest log likelihood is shown. Bootstrap test involved 1000 replicates to determine reliability of the inferred tree. The numbers at nodes represent bootstrap values. Branch lengths are scaled according to the numbers of nucleotide substitutions per site. Details of the samples are given in Table 1 and in the text. Samples 759204, 759205 and 759207 are the closest related Yamagata 2011 lineage virus sequences and taken from NCBI with the corresponding accession numbers: AB759204, AB759205, AB759207. Based on information from AB759206 (HPeV3 Yamagata 2011 lineage³⁹), the structural/capsid coding region is from nucleotide 701–3013 and the nonstructural protein coding region from 3014–7231 and inferred from that the 5′-UTR spans nucleotide 1-700 and the 3′-UTR from 7232–7334 including 12 As of the poly-A tail. A) Midpoint rooted tree and B) rooted on the closest related non-Australian sequence, AB759204.